June 2014 SDSC meeting

SDSC meeting                        sponsored by Miltenyi                                                                                                          6/25/14


Carina Torres began the meeting with highlights from the recent Cyto meeting in Ft Lauderdale.  The most interesting talks were about 40+ color flow cytometry, analyzing big data, new technologies, and shared resources.  One of the newest cell sorters was on display, the Tyto.


Jack Dunne from Miltenyi Biotec gave a great talk about the new Tyto https://www.miltenyibiotec.com/en/products-and-services/macs-flow-cytometry/flow-sorters/the-macsquant-tyto-basics.aspx.  The technology of Tyto was created by scientists at Owl Biomedical before Miltenyi acquired them.  It is a high speed sterile chip-based sorter without the need of droplets.  They utilize a MEMS (micro electro mechanical system) to open and close a valve pushing unwanted cells aside to isolate the cell of interest.  The magnetic driven valve opens and closes in 30usec.  It is a low stress cell sorter which can sort 20 million cells in 2.5hr.  The non-sorted cells are collected in a separate chamber which can be resorted or used in other assays.  It runs at 4ml/hr and can even sort whole blood diluted only 1:4.  The instrument has 8 color capability with 3 scatter parameters.  This instrument runs ~$300,000 with a cost of $50-100 per disposable chip.  The sorter will be available soon.


Stephanie Culler from Genomatica then gave a talk about using flow cytometry to develop sustainable chemicals.  They are developing strains of bacteria that can produce high yields of 1,4- butanediol (BDO).  They are using flow cytometry to monitor physiological processes, such as ATP production, redox, and viability.  Their goal is to use biomass to produce BDO instead of sugars.  These BDOs are used to make spandex, polyurethane, and polybutylene terephthalate (PBT).  Visit http://www.genomatica.com/about/ to learn more about what they do.


Don’t miss the next SDSC meeting, July 23rd




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